Roidipedia.Compound reference & reporting
05 AUG 2026
CompoundsPharmaceutical Nitrous Oxide
PharmaceuticalDissociativeNMDA antagonistInhalational anaestheticGas

Nitrous Oxide

Also known as Laughing gas · N2O · Nitrous · Nangs · Whippets · Entonox (50% mix)

Nitrous oxide (N2O, laughing gas) is an inhaled gas used for over a century as an analgesic and anaesthetic adjunct in medicine and dentistry. It acts largely as an NMDA receptor antagonist, producing brief euphoria, analgesia and dissociation. Recreational use from cream chargers is widespread, and repeated heavy use causes serious vitamin B12-related neuropathy.

01 Overview

Nitrous oxide has extensive clinical use in dentistry, obstetrics (Entonox, a 50:50 mix with oxygen) and procedural sedation. Onset and offset are very rapid because of its low blood solubility. At sub-anaesthetic concentrations it produces analgesia, giddiness and mild dissociation.

Recreationally it is inhaled from small chargers or larger tanks, giving a 30-90 second rush. The main harms are hypoxia if inhaled without oxygen, and functional B12 inactivation with heavy or chronic use, which can cause subacute combined degeneration of the spinal cord and peripheral neuropathy. Dependence with very heavy daily use is increasingly reported.

02 Mechanism

Primarily non-competitive NMDA receptor antagonism, with additional opioid and GABA-A/two-pore potassium channel actions contributing to analgesia and anxiolysis; irreversibly oxidises the cobalt in vitamin B12, inactivating methionine synthase.

03 Dosing

TierDoseRouteNotes
Recreational (single charger)1 charger (~8 g)InhaledBalloon inhalation; effect lasts under a minute.
Medical/dental analgesia30–70 % in oxygenInhaledTitrated mix with oxygen; never pure N2O for sustained use.

04 Effects

EffectMagnitudeEvidence
Euphoria and analgesiaBrief giddy euphoria with strong analgesia and altered perception.30-90 sClinical
DissociationDetachment, auditory distortion and a sense of pressure or waves at higher concentrations.briefObservational

05 Side effects

EffectSeverityFrequencyEvidenceCountermeasures
Hypoxia / asphyxiationInhaling pure N2O without oxygen, or in a confined space/bag, can cause fatal oxygen deprivation.Life-threateningUncommon but seriousObservational
B12 deficiency neuropathyRepeated or heavy use inactivates vitamin B12, causing numbness, tingling, weakness and, in severe cases, subacute combined degeneration of the spinal cord.SevereCommon with heavy useObservational
Frostbite injuryDirect inhalation from a charger or tank valve can freeze lips, mouth and airway.ModerateUncommonObservational

07 References

Nitrous oxide: neurotoxicity and clinical harms of recreational useBMJ / Pract Neurol review
Nitrous oxide as an NMDA antagonistNat Med, 1998 (Jevtovic-Todorovic)

08 Discussion0 comments

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This page is reference information, not medical advice. Doses and protocols are documented as they appear in the clinical literature and in practice — describing them is not a recommendation to use them. Countermeasures listed here are not a substitute for a physician. Legal status varies by jurisdiction and changes.